RP-HPLC determination of daidzein, genistein, daidzin and genistin in soybean extract
- VernacularTitle:RP-HPLC法测定大豆提取物中大豆苷元、染料木素、大豆苷、染料木苷的含量
- Author:
Li ZHANG
- Publication Type:Journal Article
- Keywords:
daidzein;
genistein;
daidzin;
genistin;
RP HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Object A method for the determination of daidzein, genistein, daidzin and genistin in soybean extract by RP HPLC was established Methods Zorbax SB C 18 column (4 6?15 mm, 5 ?m) was used Daidzein and genistein were separated with mobile phase Ⅰ∶methanol acetonitrile 0 1% phosphoric acid solution (30∶8∶62) Daidzin and genistin were separated with mobile phase Ⅱ∶methanol acetonitrie 0 1% phosphoric acid solution (16∶24∶60) The UV detection wavelength was 254 nm Results The average recoveries of daidzein, genistein, daidzin and genistin were 98 7%, 99 5%, 98 4%, 98 6% and the precision (RSD) were 1 4%, 0 9%, 0 9%, 1 7% (n=4) respectively Conclusion: The method was accurate, rapid and with good reproducibility
