Molecular mechanism of Helicobacter pylori resistance to clarithromycin.
- VernacularTitle:幽门螺杆菌对克拉霉素耐药的分子机制研究
- Author:
Tong SHI
;
Wenzhong LIU
;
Shudong XIAO
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
Clarithromycin resistance;
MutatiH
- From:
Chinese Journal of Digestion
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular mechanism of Helicobacter pylori (H.pylori) resistance to clarithromycin. Methods The E test was used to determine clarithromycin resistant strains of H.pylori , and PCR Restriction Fragment Length Polymorphism (RFLP) analysis for 23S rRNA domain V gene mutations. Results Of nine clarithromycin resistant stains of H.pylori , including six primary and three acquired resistant strains, eight were found to have an A to G mutation in 23S rRNA domain V at position 2144. Conclusions The results indicated that the majority (88.8%) of clarithromycin resistant isolates of H.pylori in Shanghai have an A2144G mutation in 23S rRNA domain V.
