AMPLIFICATION SEQUENCE ANALYSIS OF GnRH RECEPTOR GENE IN RAT SUBMAXILLARY GLAND
- VernacularTitle:大鼠颌下腺GnRH受体基因的体外扩增及基因序列分析
- Author:
Bing YAO
;
Weiquan HUANG
;
Rongqing ZHANG
;
Le WANG
- Publication Type:Journal Article
- Keywords:
Sequence analysis;
GnRH receptor;
RT\|PCR;
Rat submaxilla@
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective\ To study whether gonadotropin releasing hormone receptor (GnRHR)mRNA exist in rat submaxillary gland and it's gene sequence. Methods\ The total RNA isolated from rat submaxillary gland was amplified by RT\|PCR, the PCR products were identified by sequencing with Sanger's method. Results\ The specific amplified band of GnRHR mRNA was detected through agarose gel electrophoresis and gene sequence is identical to the sequence of GnRHR which has been reported in rat pituitary. Conclusion\ GnRHR can be produced by submaxillary and response for modulating biological function of submaxillary.\;
