THE EXPRESSIVE VARIETY OF NGF GENE IN INJURED SCIATIC NERVES OF RAT
- VernacularTitle:大鼠坐骨神经损伤后NGF基因表达的变化
- Author:
Xiaosong GU
;
Fei DING
;
Weilan XU
;
Dengbing YAO
- Publication Type:Journal Article
- Keywords:
NGF mRNA;
RT-PCR;
Sciatic nerve lesion;
Rat
- From:
Acta Anatomica Sinica
1989;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the expressive variety of NGF gene in injured sciatic nerves of rat. Methods For hemi-quanti- tative analysis, RT-PCR method was used in the detection of the levels of NGF mRNA in the distal stumps at 1 day and 1, 2, 4 weeks following transection of sciatic nerve. Results The 1evel of NGF mRNA was low in the normal sciatic nerve. But it was increased in the distal stump after sciatic nerve transection. The biphasic increase in NGF mRNA was characterized by a first very rapid and transient increase peaking at ist day after lesion, than a second prolonged and slow elevation starting around ist weed after lesion. Conclusion The process in NGF gene expression after sciatic nerve lesion was biphasicly increased.
