EFFECTION OF GnRH ANALOGUE ON INTRACELLULAR CALCIUM MOBILIZATION IN CULTURED RAT STOMACH SMOOTH MUSCLE CELLS
- VernacularTitle:GnRH类似物阿拉瑞林对培养的胃平滑肌细胞内钙离子浓度变化的影响
- Author:
Lei CHEN
;
Weiquan HUANG
;
Lihong LIU
;
Baozhen LU
;
Ruolei PU
- Publication Type:Journal Article
- Keywords:
GnRH analogue;
Stomach smooth muscle cell;
Ca2+;
Flue-3-AM;
Confocal microscopy
- From:
Acta Anatomica Sinica
1989;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objedctive To study the mechanism of GnRH agalogus(Alarelin)on[ Ca2 + ]; mobilization in stomach smooth muscle cells (SSMc)of rats. Methods Cells were cutured and loaded with Fluo-3-AM. [Ca2 + ]; was measured by nuomeent intensity(FL) in each cell with confocal micoopy. Results (1 ) In Hanks solution. l0-7, l0-6, l0 - 5mol L- 1 Alarelin can elevate[ Ca2 + ], its peak - resting values reached 6. 00 ?0. 50 .9. 23 ?0. 62. l8. 97 ?2. 42 respectively, which indicate that the level of [Ca2+ ]; act in an deede- pendent and time - dependent. (2) thntredly. when Alarelin was 10 4mol?Ｌ－１, its peak - resting value only reached 6. 32 ?0 .67, conpared with Alarelin l0 5m.l L－1 which was sigificantly lower(P 0. 05 ). (5 )When pertreatment with Lacidipine in Hanks solution, the effect of Alarelin l0 5mol L-1.as partly inhibited(P
