EFFECT OF Mn9202 ON 5-HT, TH AND 5-HT RECEPTOR OF CULTURED RAT STOMACH FUNDUS SMOOTH MUSCLE CELLS
- VernacularTitle:Mn9202对培养大鼠胃底平滑肌细胞的5-HT、TH及其受体的影响
- Author:
Lihong LIU
;
Qibing MEI
;
Weiquan HUANG
;
Lei CHEN
;
Feng ZHANG
;
Dehua ZHAO
- Publication Type:Journal Article
- Keywords:
Mn9202;
Lacidipine;
5-HT;
TH;
5-HTR;
Immunocytochemistry;
Stomach fundus smooth muscle cells
- From:
Acta Anatomica Sinica
1989;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of DHPs calcium antagonist Mn9202 and lacidipine on 5-HT、TH and 5-HTR of rat stomach fundus smooth muscle cells. Methods It's effects was investigated by using immunocytochemistry and image analysis of stom- ach fundus smooth muscle cells in culture and compared with cyprohetadine(antagonist of 5-HT). Results 5-HT、TH and 5-HTR im- munoreactive substances were existed in SFSMC. The results of image analysis indicated that the content of 5-HT. TH and 5-HTR were significantly lower when using Mn9202.lacidipine and cyprohetadine. Conclusion The results suggest that the rat stomach fundus smooth muscle cells possess 5-HT autocrine function, and DHPs calcium antagonist reduce the number of 5-HT receptor in the rat stomach fundus smooth muscle cells though the reduction of 5-HT sythesis, this results conform with cyprohetadine (antagonist of 5 - HT).
