Apoptosis of human fetal glomerular endothelial cell induced by neutrophil proteinase 3 from ANCA positive children with SLE
- VernacularTitle:抗中性粒细胞胞浆抗体相关抗原诱导人胎肾内皮细胞凋亡的研究
- Author:
Hong MA
;
Cuiling WANG
;
Yuan LIU
- Publication Type:Journal Article
- Keywords:
Antineutrophil cytoplasmic antibody;
Peptide peptidohydrolases;
Lupus erythematosus,systemic;
Kidney glomerulus;
Apoptosis
- From:
Chinese Journal of Rheumatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the neutrophil serine proteinase 3 (PR 3),a specific autoantigen of C ANCA,could induce human fetal glomerular endothelial cell apoptosis in vitro .Methods PR 3 was isolated from polymorphonuclear leukocytes obtained from C ANCA positive children with SLE.The cultured glomerular endothelial cells from human fetal kidneys were treated with PR 3 and apoptosis was assessed by different methods including the propidium iodide staining,DNA fragmentation analysis,and morphology observation.Results Percentage of apoptotic cells significantly increased ( P
