Effects on different concentrations of antisense oligodeoxynucleotides on human pancreatic cancer cell line PaTu8988s.
- VernacularTitle:反义寡脱氧核苷酸在不同作用时点对人胰腺癌细胞系Pa Tu8988s作用的影响
- Author:
Jianting CAI
;
Keda QIAN
;
Junshen LU
- Publication Type:Journal Article
- Keywords:
Pancreatic carcinoma;
Antisense oligodeoxynucleotides;
Time point
- From:
Chinese Journal of Digestion
1998;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of different concentrations of antisense oligodeoxynucleotides (ASODN) on human pancreatic cancer cell line PaTu8988s. Methods Human pancreatic cancer cell line PaTu8988s in exponent growth stage are adopted. We observe the effect of different time point on the PaTu8988s cell at 12、24、48 and 72 hour. Results The inhibitory rate on PaTu8988s cell line is 42.25%、66.29%、69.55%、74.58% and 66.20%、91.43%、98.18%、98.33% for ASODN concentrations of 50 ?g/ml and 100 ?g/ml at 12、24、48 and 72 hour, respectively. Conclusion The inhibitory effect of ASODN began from 12 hour and becomes more obvious at 48～72 hour. The higher the concentration of ASODN, the earlier the peak of inhibited rate.
