Effects of Octreotide and insulin on human hepatoma cell in vitro
- VernacularTitle:奥曲肽与胰岛素对人肝癌细胞影响的体外实验研究
- Author:
Qun QIAN
;
Xujun CHEN
;
Zhishu LIU
- Publication Type:Journal Article
- Keywords:
Insulin;
Octreotide;
Liver neoplasm;
Proliferation;
Inhibition
- From:
Chinese Journal of Digestion
1998;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the proliferation of human hepatoma cell can be induced by insulin and which can be inhibited by Octreotide. Methods A human hepatoma cell line BEL7402 was used in this study. Using MTT method, cell count and Flow Cytometry (FCM), the effects of insulin and/or Octreotide on the number of viable cell, its ratio, the total number of cells, cell cycle and proliferative index (PI) in phasesynchronized cells were assayed. Results The cell number, the viable cell number and its ratio in insulin (0～5 ?g/ml)treated BEL7402 cells increased, while both in basic and insulininduced ones all decreased when Octreotide 0～2 ?g/ml was used, both in dosedependent manner. 1 ?g/ml of Octreotide resulted in a reduction of PI which was increased by 5 ?g/ml of insulin. Moreover, a S cell cycle block was detectable in phasesynchronised BEL7402 cell treated with 1 ?g/ml of Octreotide. Conclusion In vitro, proliferation of human hepatoma cell can be induced by insulin, while Octreotide exerted an inhibitory effects on basic and insulininduced growth of human hepatoma cell.
