Effects of Tumor Necrosis Factor-? on Proliferation and Intracellular Free Calcium Concentration in Endothelium of Human Umbilical Vein Endothelial Cells
- VernacularTitle:肿瘤坏死因子?对脐静脉血管内皮细胞增殖和细胞内游离钙浓度的影响
- Author:
Zhen JIN
;
Ling PEI
;
Dezhi WANG
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor;
Endothelium, vascular;
Cell division;
Calcium
- From:Chinese Journal of Obstetrics and Gynecology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To Observe the effects of tumor necrosis factor ? (TNF ?) on cell proliferation and Intracellular free calcium concentraction ([Ca 2+ ]i) in endothelium of human umbilical vein endothelial cells (HUVEC) and investigate the pathogenesis of pregnancy induced hypertension syndrome (PIH). Methods Confluent monolayer of HUVEC was directly incubated with TNF ? at following final concentrations: 500, 1 000, 2 000 U/ml for 24 hours. The percentages of different cellcycles and [Ca 2+ ]i were measured by flow cytometry and fluorospectrophotometry. Results Incubated with TNF ?, the endothelial cells were elongated and transformed into fibroblast like cells. Border of nucleus was sharp, clarity, and cells were in regular shape. But there were abnormal granules in cytoplasma and some cells detached at the concentrotion of 2 000 U/ml of TNF ?. Stimulated by TNF ?, the percentage of cellcycles from phase G 0+G 1 to S and G 2+M decreased significantly and it was dose dependent. [Ca 2+ ]i increased significantly and dose dependent as well. Conclusion TNF ? may injure endothelium directly and inhibit its proliferation and repair through the changes of [Ca 2+ ]i level. It may play an important role in the pathogenesis of PIH.
