Quantitative Determination of Chlorogenic Acid in Xiaoer Jiebiao Oral Liquid by HPLC
- VernacularTitle:RP-HPLC法测定小儿解表口服液中绿原酸的含量
- Author:
Min LU
;
Huiwu ZHOU
- Publication Type:Journal Article
- Keywords:
Xiaoer Jiebiao Oral Liquid/chemistry;
chlorgenic acid/analysis;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The content of chlorogenic acid in Xiaoer Jiebiao Oral Liquid was determined by HPLC.ODS column was applied.Mobile phase was methanol_1% acetic acid(25∶75),wavelength of detector was 328nm.Samples were extracted with methanol on ultrasonic vibrator.In this method,the linera range of chlorogenic acid is 0.232 ~ 1.16 ?g,the correlation coefficient is 0.9994 ,and the mean recovery is 98.44%. The results showed that this method is simple,quick and reproduciple.