Expression, induction and mutation of connexin genes in human gastric cancer
- VernacularTitle:细胞连接蛋白基因在胃癌中的表达、诱导及突变研究
- Author:
Shourong SHENG
;
Fue LI
;
Yang LIU
- Publication Type:Journal Article
- Keywords:
Gastric cancer;
Connexin gene;
Gene expression;
Induction;
Gene mutation
- From:
Chinese Journal of Digestion
1996;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of connexin (Cx) genes and the effects of a variety of inducers on the expression changes of Cx and mutations of Cx coding sequences in human gastric cancer. Methods Northern Blot, RT PCR and PCR single strand conformational polymorphism were used. Results 1.There were regular expression patterns of Cx genes in human normal gastric epithelium, paracancerous tissues and gastric cancers. 2.RA and DMSO can induce the expression of Cx43 in gastric cancer and decrease of Cx46 expression by RA and TPA induction. 3.There was no any mutation in Cx43 coding sequences. Conclusion 1.Cx32 gene may be a specific gene in genome of gastric epithelium constructing gap junctional intercellular communication. 2.Cx43 is a inducible gene in gastric cancer cells. 3.The down regulation of Cx43 expression is not caused by mutation of Cx coding sequences in human gastric cancers. 4.The authors proposed a hypothesis on the variation of expression of Cx genes in human gastric cancer.
