PREPARATION OF A RNA PROBE FOR HUMAN TYROSINE HYDROXYLASE
- VernacularTitle:人酪氨酸羟化酶RNA探针制备的研究
- Author:
Shuting LI
;
Hui YANG
;
Jinlu ZHANG
;
Qing CAI
;
Qunyuan XUN
- Publication Type:Journal Article
- Keywords:
Human tyrosine hydroxylase;
pGEMTH 2;
Dig labeled RNA probes;
Dot blot hybridization
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective\ To prepare a digoxingen labelled cRNA probe for human tyrosine hydroxylase(hTH 2).Methods\ Using molecular cloning techniques the recombinant plasmid pGEMTH 2 was constructed.According to analysis of restriction endonucleases,the pGEMTH 2 contained the DNA fragment of hTH 2 gene and inserting sit preparation was correct for cRNA probe.From recombinant plasmid pGEMTH 2 prepared cRNA probe was identified by dot blot hybridization.Results\ The dot blot hybridization showed that the positive reaction sites were purple blue.Conclusion\ This probe prepared in present study was sensitive and reliable.This probe might provide a tool for identifying the effect of gene therapy in animal model of Parkinson disease.
