Determination of Fraxetin A and Fraxetin B in Cortex Fraxini by HPLC
- VernacularTitle:HPLC法测定秦皮中秦皮甲素、秦皮乙素的含量
- Author:
Zhong JI
;
Kou WANG
- Publication Type:Journal Article
- Keywords:
HPLC, Cortex Fraxini, fraxetin A, fraxetin B
- From:
Chinese Traditional Patent Medicine
1992;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
The determination of fraxetin A and fraxetin B in Cortex Fraxini have been carried out by HPLC on the stationary phase:octodecyl-silicohydride bonded silica gel column. The mobile phase was methanol-water(24:76). The detection wavelength was at 348nm. There was a good linear relationship in a concentration range of 16?g/ml~176?g/ml. The correlation coefficients were 0. 9995 and 0. 9994,respectively. The recovery of the added sample were 99. 1 ?1. 5% (n=5) for fraxetin A and 99.8?2. 5%(n=5) for fraxetin B. This method showed 0. 8% of within-day accuracy and 6. 2% of between-day accuracy,involving the advantages of simplicity,quickness and accuracy.
