Determination of Free Lysine in Queen Bee's Embryo Preparation by Spectrophotometry
- VernacularTitle:分光光度法检测蜂皇胎制品中游离赖氨酸含量
- Author:
Liling CAI
;
Shizhong ZHANG
;
Mousheng ZHENG
- Publication Type:Journal Article
- From:
Chinese Traditional and Herbal Drugs
1994;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Free lysine in a preparation of queen bee's embryo was isolated using cation exchange resin- The Q-aminoand carboxyl group of lysine was complexed with cupric ion and the E-amino group reacted with FDNB(Fluoro-dinitrobenzene). After acid treatment and extraction with ether,the absorbance of the solution at 395nm was determined by spectrophotometry. Variation coefficient of the determination was less than 6% and the recovery of lysine was 95 % ~ 110 %.
