Quantitative Determination of Hyodeoxycholic Acid in Calculus Bovis Antidote Tablet by TLC Scanning
- VernacularTitle:薄层扫描法测定牛黄解毒片中猪去氧胆酸的含量
- Author:
Shaohuei WAN
;
Zhaotao LI
;
Shaohui WAN
- Publication Type:Journal Article
- From:
Chinese Traditional and Herbal Drugs
1994;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Hyodeoxycholic acid in Calculus Bovis Antidote tablet was determined quantitatively by dual wavelength TLC scaning. The experiment was carried out on silica gel-G plates with chloroform -ether-glacial acetic acid (2:2:1) as the mobile phase, and ethanol solution of phosphomolybdic acid (10% ) as developer, at ?s=700nm,?R= 450nm. Recovery was 103. 4% and RSD=3. 2% (n=5 ). This method is accurate and convenient It can also be used for quantitative analysis of other preparations containing hyodeoxycholic acid
