Application of Thin Layer Chromatograph-Fluorescent Method in Quality Control of Herbal Preparations
- VernacularTitle:薄层刮板-荧光分析法在中药复方制剂质量控制中的应用
- Author:
Ling LI
;
Mingzhu LIU
;
Weiming XU
- Publication Type:Journal Article
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
A thin layer chromatograph-fluorescent method was used to assay content of scopoletin in Erycibe obtusi folia Benth.. The method showed that it not only can minimize analytical errors in thin layer scan and column contamination in HPLC,but also eliminated mutual interference of components in the preparation. It provides a good assay technique for quality control of hebal preparations. The method was carried out on thin layer plates of silica gel H(40?)with benzene-ether (1. 1: 1 )as the developer,at an excitation spectrum of 347nm,fluorescence spectrum of 420nm and a temperture of 25??1℃.The method showed good linearity(r= 0. 9998, n = 5)in the range of 0.08~0. 40?g/?l of scopoletin and the average recovery was 98. 52 % ~ 102. 67 % with RSD
