Studies on Effective Composition of Pinecone V.Determination of Lignin in Polysaccharides of Cone of Pinus koraiensis by TLC-UV method
- VernacularTitle:松果有效成分的研究V.TLC-UV法测定红松果多糖中木脂素含量
- Author:
Haozhi LI
;
Yongjlln LU
;
Xiaobin YUAN
- Publication Type:Journal Article
- From:
Chinese Traditional and Herbal Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
A TLC-UV method for the determination of lignin in polysaccharides of cone of Pinus koraiensis was reported. Active lignin was obtained by acid hydrolysis,and developed on GF-254 TLC-Plate by mixed Solventof dich1oro methane-ethylether. UV absorbance of the eluate was determined at 23Onm. The concentration oflignin was linearly related to absorbance within the range of 2~70pg/ml. The recovery was 100. 2% ~ 101.4% and RSD was 6. 5 %.
