Tissue Culture of Thickstemen Gentian(Gentiana crassicaulis)and Plant Regeneration Through Somatic Embryogenesis
- VernacularTitle:粗茎秦艽组织培养及再生植株
- Author:
Yuling MENG
;
Jingfen JIA
- Publication Type:Journal Article
- From:
Chinese Traditional and Herbal Drugs
1994;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Callus of Gentiana crassicaulis was initiated from hypocotyl and cotyledon on MS medium supplemented with 2mg/L 2.4-D and 0.5 mg/L BA.Induction frequencies of callus were 100%.Somatic embryos were formed on MS medium containing 2mg/L BA,3mg/L ZT, 1mg/L NAA,3mg/L GA,three time MS organics and vitamins,6% sucrose and 500 mg/L LH and three time FeSO4 (Na2EDTA) .40% Callus produced somatic embryos.It developed into complete plants on hormon-free MS medium.
