Determination of Flavonoids in Leaf Extract Preparations of Ginkgo (Ginkgo biloba) by HPLC
- VernacularTitle:高效液相色谱法测定银杏叶制品中总黄酮
- Author:
Songqing LIU
;
Xianzhe TANG
;
Wenxiu MA
- Publication Type:Journal Article
- From:
Chinese Traditional and Herbal Drugs
1994;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Quantitative HPLC method was developed for the determination of flavonoids in extract preparations of leaves of Ginkgo biloba L. YWG-C13 column was used with a mobile phase of methanol-water-glacial acetic acid (40: 57.5 : 2.5, V/V ) and detected at 254nm.The flow rate was 1.0ml/min. Ten Peaks were observed. Rutin was used as external standard and the calibratiott cirve was linear over the rang of 0.5~2.5?g (r =0.9996 ). The extraction recovery was 104.2% (RSD = 3.3% ) .Compared with the res ilts of chemical colorimetric analysis, the method has a better reprod icibility and more information about the flavonoids in G. biloba can be obtained.
