Studies on Effective Composition of Pinecone Ⅳ.Determination of Acid Polysaccharides in Polysaccharides of Cone of Korean Pine (Pinus koraiensis)
- VernacularTitle:松果有效成分的研究Ⅳ.红松果多糖中酸性糖的含量测定
- Author:
Haozhi LI
;
Tiejun GUO
- Publication Type:Journal Article
- From:
Chinese Traditional and Herbal Drugs
1994;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Acid polysaccharides in pinecone were determined quantitatively by sulfuric acid-carba zole colorimetry and the influence of amount of sulfuric acid carbazole, reaction temper ature and time forcolor-reaction were investigated. The maximum abstrption was found to be at 520nm. The absorbance was a linear function of concentrations between 0 and 7 ?g/ml acid polysaccharides (r = 0.9999, n = 7). Analytical recovcry was 91.0%～99.8% . RDS was 1.3%～2.3% (n = 6 ). The minimum detectable concentration was 0.05?g/ml.
