IMMUNOCYTOCHEMICAL STUDY OF GONADOTROPIN-RELEASING HORMONE (GnRH) NEURONS IN DISSOCIATED CULTURES OF NEWBORN RAT HYPOTHALAMUS
- VernacularTitle:大鼠下丘脑细胞培养中GnRH神经元的免疫细胞化学研究
- Author:
Xiangmin YAO
;
Tianbao SONG
- Publication Type:Journal Article
- Keywords:
Gonadotropin-releasing hormonal neuron;
Hypothalamus;
Neuronal culture;
Immunocytochemistry;
Newborn rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Gonadotropin-Releasing Hormone (GnRH) immunoreactive neurons in dissociated cell culture from newborn rat hypothalamus were investigated on days 1,3,5,and 7 in vitro by means of the im-munocytochemical method. The results showed that GnRH was expressed in the first day of culture. GnRH neurons accounted for 12.1-14. 8% of the total neurons in culture,and they were mainly bipolar in type. There were growth cones on the end of GnRH processes. Various patterns of intercellular contacts between GnRH neurons and between GnRH and other neurons were also observed. These findings indicate that the cultured GnRH neurons exhibit the morphological and functional characteristics of the GnRH neurons in vivo,and serve as morphological evidence for pulsatile secretion of GnRH and its regulation.
