CHARACTERIZATION,ISOLATION AND CULTURE OF OSTEOCLASTS FROM NEONATAL RAT LONG BONES
- VernacularTitle:大鼠破骨细胞体外分离培养和鉴定
- Author:
Weizhen ZHANG
;
Shifeng YU
;
Linfan ZHENG
- Publication Type:Journal Article
- Keywords:
Osteoclast;
Bone resorptioa;
Histochemistry;
Rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Osteoclasts were isolated mechanically from neonatal rat long bones and cultured on glass or devitalized human cortical bone slices. Both morphological and functional studies showed that the isolated cells were of typical osteoclasts, because; 1. they attached rapidly on the matrix and had pseudopodial activity when cultured on glass;2. they had multinuclei,most of them contained 3-10 nuclei;3. calci-tonin and clacitonin gene related peptide directly inhibited the cellular activity; 4. they showed high acid phosphatase activity; 5. resorption lacunae could be found when the cells cocultured with devitalized bone slices.
