Determination of Bis-(5-formyl-furfuryl)-ether and 2,4-Dihydroxy-6-methoxy-3-methyl-acetophenone in the Root of Yuexiandaji(Euphorbia ebracteolata) by HPLC
- VernacularTitle:HPLC法测定月腺大戟根中狼毒甲素及狼毒乙素的含量
- Author:
Kuijun ZHAO
;
Guojun XU
;
Rongluan JIN
- Publication Type:Journal Article
- From:
Chinese Traditional and Herbal Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
An HPLC method for the quantitative analysis of bis (5-formyl-furfuryl ) -ether an d 2, 4-dihydroxy-6- methoxy-3-methyl- acetophenone in Chinese drug,Langduc (Euphorbia ebracteolata)was developed. The solvent system used was methanol-water-10% acid (45 : 55 : 2 ) (adjusted to pH 5.4 by 10% ammonia ) on ODS columm at 289nm.Tetrahydro palmatine was used as an internal standard. The operation can be completed in 30min.This method is sensitive,simple and accurate with good reproducibility.It can be applied to the quality control of the crude drug of E. ebracteolata.
