A CHEMICAL NEUROANATOMICAL STUDY ON THE GABA-CONTAINING NEURONS IN THE ANTERIOR HORN OF THE RAT SPINAL CORD
- VernacularTitle:大白鼠脊髓前角内含片氨基丁酸神经元的化学神经解剖学研究
- Author:
Qiuyun CAI
;
Changgeng ZHU
- Publication Type:Journal Article
- Keywords:
?-Aminobutyric acid;
Spinal cord;
Immunoelectron microscopy;
Immunohistochemitry;
Rat
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The distribution of GABA-containing neurons in the anterior horn of L_(4～5) segments of rat spinal cord and their relationship with somatic efferent were studied by combined method of HRP and immunocytochemistry and immunoelectron microscopic method. The results showed that under light microscope, the GABA-immunoreactive cell bodies and terminals were seen in all layers of the anterior horn including Rexed′s layer IX which located in the anterolateral part of the anterior horn. The GABA-positive neurons had round or triangle cell body with many processes and could be divided into two (large and medium) types. Under electron microscope, GABA-immunoreactive products appeared as small granular deposits located in perikarya, dendrite and axon. In axon terminals the immunoreactive products located at periphery of the synaptic vesicles and on the outer membrane of the mitochondria. The GABA-positive dendrites received symmetrical afferent synapses from GABA-positive or negative axon terminals. The combined method of HRP and immunocytochemistry showed that in Rexed′s layer IX there were HRP single labeled neurons, GABA single labeled neurons and HRP/GABA double labeled neurons. The double labeled neurons accounted for 79% of total HRP labeled cells. Above mentioned results first identified the neurons in Rexed′s layer IX of the anterior horn contain GABA which participate in somatic efferent and receive autoregulation from GABA-neurons and nonGABA-neurons at synaptic level.
