Alterations of p16INK4A Gene in Korean Squamous Cell Carcinoma and Basal Cell carcinoma of the Skin.
- Author:
Chang Duk KIM
1
;
Chang Wook KIM
;
Sung Il SUH
;
Sang Pyo KIM
;
Kyu Suk LEE
Author Information
1. Department of Dermatology, Keimyung University School of Medicine, Taegu, Korea. franzes@dsmc.or.kr
- Publication Type:Original Article
- Keywords:
Squamous cell carcinoma;
Basal cell carcinoma;
p16INK4A;
Methylation
- From:Korean Journal of Dermatology
2004;42(7):821-824
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The inactivation of the cyclin dependent kinase 4 inhibitor p16INK4A may be caused by deletion, mutations or promoter hyper-methylation. Recently, DNA methylation of p16INK4A promoter has been shown to suppress of p16INK4A gene transcription in various tumors. OBJECTIVE: We analyzed the gene status of p16INK4A in squamous cell carcinoma (SCC) and basal call carcinoma (BCC) of the Korean skin to investigate whether the inactivation of the gene participated in carcinogenesis. METHODS: We examined 12 SCC and 3 BCC cell lines using PCR to detect homozygous deletion of chromosome 9p21 region and methylation-specific PCR. RESULTS: Deletion of the p16INK4A gene were not, but methylation of the p16INK4A promoter region was common in SCC of the skin. CONCLUSION: These results suggest that de novo methylation of the p16INK4A promoter region seems to play an important role in the pathogenesis of SCC.
