HPLC Determination of Main Nucleoside and Nucleic Acid Base in Mycelia of Cordyceps
- VernacularTitle:冬虫夏草发酵菌丝体中主要核苷及核酸碱基的HPLC测定
- Author:
Lixia JIANG
;
Fang LIU
- Publication Type:Journal Article
- Keywords:
Mycelia of Cordyceps;
nucleoside nucleic acid base;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Six nucleosides and base components in Four products of Mycelia of cultivated Cordyceps were determined. The comparison of extraction solvents and recovery test of the adenosine components were emphasized. The effective extraction-rate by water,as an extraction solvent, was higher than that by 50% alcohol. The addition of 0. 1% sodium azide into water could not only preserve putrefaction,but also make a contribution to the stability of nucleoside components in the liquid sample. When the extraction was carried out by ultrasonic at room temperature,the recovery rate of the added sample was 99. 8%.
