Content Determination of Paeoniflorin in Blood Plasma with Reversed-phase High-efficiency Liquid Chromatography
- VernacularTitle:反相高效液相色谱法测定血浆中芍药甙的含量
- Author:
Xuehua JIANG
;
Shiduan LIU
- Publication Type:Journal Article
- Keywords:
reversed-phase high-efficiency liquid chromatography, paeoniflorin, concentration of blood plasma
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The method, established for analysis of paeoniflorin in blood plasma, appeared a good linear relationship(r=0.9998). Using meta-hydroxybenzoic acid as internal standard and calibration factor combined, the quantitation precision of method could be raised. The result of determination of calibration factor conformed with the chromatography prescriptions in "Pharmacopoeia of China" (edited in 1990). The average recovery reached up 97.13％. The minimum detection amount was 5.6ng. The coefficients of within-day and between-day variation were 0.39％ and 1.17％, respectively. This method is suitable for paeoniflorin analysis of in vivo sample . At present,the in vivo absorption of Baihegujintang Granules (containing Radix Paeoniae alba or Radix Paeoniae rubra) by rabbit is preliminarily investigated utilizing this method.
