QUANTITATIVE ANALYSIS OF SUBSTANCE PCONTAINING NERVE FIBERS AND CELLS IN THE NUCLEUS OF DORSOLATERAL FUNICULUS OF THE RAT
- VernacularTitle:含P物质神经纤维和细胞在大鼠脊髓背外侧索核区的定量分析
- Author:
Li SHEN
;
Lei YANG
;
Luxi XU
- Publication Type:Journal Article
- Keywords:
Spinal cord;
Nucleus of the dorsolateral funiculus;
Substance P;
Rat
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Using immunocytochemical method and image analysis computer system, quantitative analysis of substance P (SP) containing nerve fibers and neurons in the nucleus of dorsolateral funiculus (NDLF) was studied in the rat spinal cord. Under light microscope, in the white substance of the whole length of the spinal cord, a concentrated triangular or cuneiform area of SP-immunoreactive positive fibers and cells could be observed in the NDLF region. Using the image anlysis computer system (IBAS 2000) the outline of the whole SP stained area in the NDLF was drawn with a light point of the Mouse and this whole area was measured and named as the reference area (REFAREA). We selected the optimal gray level of the image in NDLF region by the Interactive Segment as the threshold. The sum of the SP labelled areas was called the field area (FAREA). The ratio of the FAREA to REFAREA (AREA%) represented the percentage of density to area of the SP labelled substance in NDLF. The mean value of REFAREA, FAREA, and AREA% are: 47931.38?m~2, 21995.25?m~2, and 45.89% in the cervical segments; 31575.15 ?m~2, 7646.85?m~2, and 24.22% in the thoracic region; 26889.89?m~2, 8470.66?m~2, and 31.50% in the lumbar and sacral segments, respectively. In short, according to the density of SP positive fibers and neurons in NDLF and the size of their distributive area, the sequence of density and size from highest to lowest is: cervical, lumbar, sacral and thoracic segments, with the lowest one at thoracic 8-10 segments.
