HPLC Determination of Bufogenin in Liuying Pills
- VernacularTitle:六应丸中脂蟾毒配基的HPLC测定法
- Author:
Yunxing WANG
;
Junxian YUAN
- Publication Type:Journal Article
- Keywords:
HPLC determination, bufogenin, Liuying Pills,calibration curve method of external Peak area
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Reversed high-pressure liquid chromatography was applied in content determination of bufogenin in Liuying Pills. The sample was extracted with chloroform. Using the calibration curve method of external peak area for quantitation, ODS as chromatographic column, 60％ methanol as mobile phase, and detection wavelength at 298nm, the recovery reached 97.7％(CV=0.5％).
