A DOUBLE LABELED IMMUNOELECTRON MICROSCOPIC STUDY OF NEUROTENSINERGIC AND SUBSTANCE P-ERGIC STRUCTURES IN THE RAT ARCUATE NUCLEUS
- VernacularTitle:大鼠弓状核内神经紧张素能和P物质能阳性结构的免疫电镜双标记研究
- Author:
Xijing ZHANG
;
Changgeng ZHU
;
Hantao LIU
;
Jinhuo LU
- Publication Type:Journal Article
- Keywords:
Arcuate nucleus;
Neurotensin;
Substance P;
Immunoelectron microscopy;
Double labeled method
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The distributions of neurotensin(NT) and substance P(SP) in the arcuate nucleus of rat hypothalamus have been studied by means of double labeled pre-embedding immunoelectron microscopic technique. It was observed that there were SP- and NT-containing dendrites, perikarya and axons in the arcuate nucleus. SP- and NT-containing dendrites and axons received asymmetric afferent synapses from immunonegative axons. SP-positive axonal terminals established symmetric axo-somatic and axo-dendritic synapses with immunonegative perikarya and dendrites as well as symmetric axo-somatic synapses with NT-positive perikarya. The results of this study directly indicate for the first time that the NT-ergic neurons in rat arcuate nucleus receive innervation from SP-ergic neurons, and provided an ultrastructural evidence for the synaptic regulation of the neuroendocrine of the hypothalamus.
