EFFECT OF RABBIT RETICULOCYTES 5srRNA ON DNA SYNTHESIS OF MOUSE MYELOMA CELLS SP2/0
- VernacularTitle:兔网织红细胞5srRNA对小鼠骨髓瘤细胞SP2/0DNA合成的影响
- Author:
Yan XU
;
Xue HU
;
Peixuan ZHANG
;
Shepu XUE
- Publication Type:Journal Article
- Keywords:
Reticulocytes 5srRNA;
DNA synthesis;
Autoradiography;
~3H-TdR
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
5srRNA was isolated and purified from the reticulocytes of rabbit. It labeled with ~(125)I, then incubated with mouse myeloma cells (SP2/0). By autoradiography it was observed that ~(125)I-SsrRNA could pass into the nuclei of the cells. In a separate experiment, it showed that the incorporation rate of ~3H-TdR into nuclei of SP2/0 after incubation with 5srRNA was decreased as compared with that f control group, hence the result indicates that 5srRNA inhibits DNA synthesis of the SP2/0 cells and it seems to play a role in the regulation of gene expression through its hybridization with DNA sequences of the SP2/0 cells. Thus it is likely that 5srRNA might act as "erythroid denucleation factor".
