A STUDY OF THE EFFECTS OF PROTECTANTS WITH DIFFERENT CONCENTRATION OF GLYCEROL AND SUCROSE ON CRYOPRESERVATION OF HUMAN SPERMATOZOA
- VernacularTitle:不同浓度的甘油蔗糖保护剂对人类精子冻存效果的研究
- Author:
Yanrong WANG
;
Ningfang MA
;
Wenxian ZHOU
;
Xiu CUI
- Publication Type:Journal Article
- Keywords:
Human spermatozoa;
Semen cryopreservation;
Glycerol;
Sucrose;
Cryoprotective media
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The effects on frozen-thawed sperm survival rate of 16 different cryoprotective media containing different ratio of glycerol (0%, 2.5%, 5% and 7%) and sucrose (0,25,50 and 100 mmol/L) were compared. The results showed that glycerol-sucrose cryoprotective media had better effect on cryoprotection of spermatozoa than that of traditional glycerol protective medium, and appropriately increasing the concentration of sucrose and decreasing the concentration of glycerol could improve sperm motility, and especially benefit to preserve sperm linear motility at 12h postthawed. Using 5% glycerol combined with 50 mmol/L sucrose as cryoprotective medium, the sperm survival rate at 0, 6, 12h postthawed was 85.38%, 51.22%, 33.38%, respectively, the linear moving sperm survival rate was 83.74%, 33.33%, 18.38% respectively.
