THE PROLIFERATION OF HEPATOCYTES AND IMMUNOHISTOCHEMICAL STUDY OF ALPHAFETOPROTEIN AND ALBUMIN AFTER SUBTOTAL HEPATECTOMY AND DGALACTOSAMINE INDUCED HEPATIC INJURY
- VernacularTitle:大鼠肝大部切除与D-氨基半乳糖肝中毒后肝细胞增殖及甲胎蛋白和白蛋白免疫细胞化学研究
- Author:
Cuiping ZHONG
;
Lingzhong CHENG
- Publication Type:Journal Article
- Keywords:
Subtotal hepatectomy;
D-galactosamine hepatotoxicity;
Hepatocyte proliferation;
Alpha-fetoprotein;
Albumin;
Immunocytochemistry
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Male Wistar rats were performed 70% partial hepatectomy (PH) or 1.5mg/g body weight D-galactosamine injection (Gal) intraperitoneally. From the 1st to the 7th day after treatment, the serum concentration of alpha-fetoprotein (AFP) and albumin (ALB) was measured and the liver sections were stained with routine and immunohistochemical methods and observed with light and electron microscopy. The mitotic index (MI) reached a maximum level (1.5%) on the 2nd day after PH, while the Gal group showed a lower (0.8%) and delayed (on the 3rd day) MI peak. On the 2nd and 3rd day after PH, AFP positive hepatocytes were predominantly observed in the periportal area and ALB positive cells mainly distributed around the central veins. In Gal group, AFP was detected both in hepatocytes near the necrotic areas and particularly in some special small cells which had not been seen in PH group. These small cells seemed to be able to transform into typical hepatocytes. Intracellular localization of AFP and ALB was observed in the perinuclear space, RER, and Golgi complex. Serum AFP and ALB levels in both groups presented reciprocal changes from the 1st to the 7th day after treatment.
