PREPARATION OF MONOCLONAL ANTIBODY AGAINST PROTEIN KINASE A AND ITS IMMUNOCYTOCHEMICAL LOCALIZATION IN NORMAL AND MALIGNANT CELLS
- VernacularTitle:依赖于环腺苷酸蛋白激酶的单克隆抗体制备及其在正常和恶性细胞内的免疫细胞化学定位观察
- Author:
Jiachun FANG
;
Yongjin SHI
;
Minying NIU
;
Yunyan LIANG
;
Menglian ZHAO
;
Daishu WANG
- Publication Type:Journal Article
- Keywords:
Protein kinase A;
Monoclonal antibody;
Immunofluorescent localization;
Normal and malignant cells
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
A rat monoclonal antibody of the IgG1 class, McAb B5A8, specific for the cAMP-dependent protein kinase(PKA) was produced. Western blot analysis revealed specific binding of the antibody to protein of 52-56 kd.The affinity-purified McAb BSA8 was labeled with FITC. Immunofluorescent localization of PKA was examined in human fibroblasts, gastric cancer cell line (MGc 80-3)and EAC cells. The distribution of PKA on the cytoplasmic microtubule network, Golgi region and nucleoli were observed. PKA was localized in the nuclear region in G2 phase of synchronized MGc 80-3 cells, and it was only found around the nuclei of MGc 80-3 cells which were incubated with DBcAMP. The changes in the distribution of PKA in cells are discussed.
