STUDY OF THE INTERACTIONS BETWEEN CULTURED NEURAL CELLS FROM CERTAIN NUCLEI OF THE BASAL FOREBRAIN AND RETINA OF NEWBORN RAT
- VernacularTitle:初生大鼠前脑基底某些神经核区和视网膜的神经元在培养中相互作用的研究
- Author:
Minghua ZHOU
- Publication Type:Journal Article
- Keywords:
Basal forebrain;
Neural cell;
Culture;
Nerve growth factor;
MTT colorimetric microassay
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
In order to study the cause of mental deterioration and increase of plaques and tangles in the basal forebrain in Alzheimer's disease, we have used isolated neural cells from the nuclei of amygdala (A), the hippocampus(H), the nuclei of medial septum (S), and the basal nuclei of Meynert (M) of basal forebrain, and the retina (R) of newborn rats, to study the effect of cellular interactions on the growth activity of these cells. The growth activity was measured quantitatively using MTT colorimetric microassay method. The MTT results revealed that, after 3 days of culture, the most active cell growth were found in mixed cultures of A+H and H+R reaching up to 212％ and 270％ of their respective individual control cultures (p
