IMMUNOCYTOCHEMICAL PROPERTIES OF NEURONS IN THE CENTRAL NUCLEUS OF THE AMYGDALA PROJECTING TO THE OVAL NUCLEUS OF THE BED NUCLEI OF THE STRIA TERMINALIS IN THE RAT: A COMBINED FLUOROGOLD RETROGRADE TRACT-TRACING AND IMMUNOFLUORESCENCE STAINING STU
- VernacularTitle:大鼠中央杏仁核-卵圆核投射神经元的免疫细胞化学特征——荧光金逆行追踪结合免疫荧光双标记法研究
- Author:
Zhongsheng HAN
;
Gong JU
- Publication Type:Journal Article
- Keywords:
Bed nuclei of the stria terminalis;
Oval nucleus;
Central nulceus of the amygdala;
Tract-tracing;
Immunofluorescence;
Double labelling;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The central nucleus of the amygdala(Ce) is heterogeneous in cyto- and chemoarchitecture, and contains over a dozen neuropeptides. Our recent works suggested that there were reciprocal connections between the Ce and the oval nucleus (Or) of the bed nuclei of the stria terminalis (BST). The present experiment was designed to study the immunocytochemical properties of the Ce neurons projecting to the Ov by the fluoro-gold(FG) retrograde tract-tracing method combined with indirect immunofluorescence staining in male Sprague-Dawley rats.It was found that the neurons in the Ce were labeled densely following injection of FG into the Ov, and some FG labeled neurons were restained by indirect immunofluorescence procedures using antisera to corticotropin-releasing factor (CRF), neurotensin (NT), M-enkephalin(M-ENK) or cholecystokinin(CCK). The findings suggest that the CRF-, NT-, M-ENK- and CCK-containig neurons in the Ce project to the Ov.
