SERUM-FREE CULTURE OF SSV-NIH3T3 CELLS
- VernacularTitle:SSV-NIH3T3细胞的无血清培养
- Author:
Yasheng GAO
;
Jingsheng TIAN
;
Jing PANG
- Publication Type:Journal Article
- Keywords:
Serum-free culture;
SSV-NIH_3T_3 cells;
Platelet-derived growth factor
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The establishment of a serum-free culture medium (SFM) for SSV-NIH3T3 cells is reported in this paper. In this medium DMEM/Ham's F12 is the fundamental element in basal medium, insulin and undefatty bovine serum albumin are key elements among the supplements. In this SFM SSV-NIH3T3 cells grow well, they keep the ability of secreting platelet-derived growth factor-like material into culture medium and causing tumor growth in nude mice.
