PROJECTIONS OF CRF- AND NT-CONTAINING NEURONS IN THE OVAL NUCLEUS OF THE BED NUCLEI OF THE STRIA TERMINALIS TO THE POSTERIOR PART OF THE LATERAL HYPOTHALAMUS IN THE RAT: A COMBINING FLUOROGOLD RETROGRADE TRACT-TRACING WITH IMMUNO CYTOCHEMISTRY STU
- VernacularTitle:卵圆核内促肾上腺皮质激素释放因子和神经降压素免疫反应阳性神经元向后部下丘脑外侧区投射——荧光金逆行追踪结合免疫荧光双标记法研究
- Author:
Zhongsheng HAN
;
Gong JU
- Publication Type:Journal Article
- Keywords:
Bed nuclei of the stria terminalis;
Oval nucleus;
Lateral hypothalamic area;
Tract-tracing;
Immunocytochemistry;
Double labelling;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
In our recent work it was found that corticotropin-releasing factor (CRF) and neurotensin(NT) were coexistent in the neurons of the oval nucleus (Ov) of the bed nuclei of the stria terminalis (BST) and there were reciprocal connections between the Ov and the lateral hypothalamic area(LHA).In the present experiments fluoro-gold (FG) retrograde tract-tracing technique combined with indirect immunofluorescence staining was used to investigate the chemical properties of the Ov neurons projecting to the LHA in the male Sprague-Dawley rats. It was found that after injecting of FG into the posterior LHA the retrogradely labeled neurons on the Ov were positively stained with antisera to CRF or NT. The present study proves that a part of CRF- and NT-containing neurons in the Ov project to the posterior LHA.
