PREPARATION OF MONOCLONAL ANTIBODIES TO HUMAN TYPE Ⅲ COLLAGEN AND THEIR LOCALIZATION IN NORMAL TISSUES
- VernacularTitle:抗人Ⅲ型胶原单克隆抗体的制备与正常组织定位
- Author:
Minghua ZHU
;
Yanfang LIU
- Publication Type:Journal Article
- Keywords:
Type Ⅲ collagen;
Monoclonal antibody;
Antigenic determinant;
Immunohistochemistry
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Monoclonal antibodies, CAb-15 and CAb-16, were produced by using of spleen cells from Balb/c mice immunized with cell suspension of fresh hepatoma tissues with mouse myeloma Sp 2/0 cells. Western blotting showed that the antigen determinant of CAb-15 was situated on the ?_1 [Ⅲ] polypeptide chains and that of CAb-16 on the triple helical domain. The monoclonal antibodies were also used for immunohistochemical localization by ABC method in formalin-fixed and paraffin-embedded sections of normal tissues from liver, kidney, aorta, skin, cartilage, placenta, stomach, breast and granulation tissues. The results showed that these antibodies are useful in immunohistochemical study for collagen.
