A MORPHOLOGICAL STUDY ON TWO TYPES OF ALVEOLAR MACROPHAGES FROM NORMAL RAT LUNG
- VernacularTitle:大鼠肺泡巨噬细胞类型的形态学研究
- Author:
Ming YU
;
Longmei WA
- Publication Type:Journal Article
- Keywords:
Alveolar macrophage;
Differential interference contrast microscope;
Electron microscope;
Enzyme histochemistry;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Alveolar macrophages (AM) were studied by means of phase contrast microscope, differential interference contrast microscope, SEM and TEM. The content of AcP, LDH, SDH of the AM was measured by MPV3-TSA microspectrophotometerimage analytical instrument. Under phase contrast microscope, two different types of AM can be distinguished, i. e, the light cells and the dark cells. The two types of cells are spherical and flat in shape under differential interference contrast microscope. The observation with SEM and TEM showed that the spherical cells possess more filopodia and more lysosomes in cytoplasm, but the flat cells possess more lamellar podia. Quantitative cytochemistry demonstrated that the content of AcP, LDH, SDH in spherical cells are much more than that of flat cells. The results suggest that there are two types of alveolar macrophages exist in normal rat lung.
