DETERMINATION OF ANTICANCER DRUG SENSITIVITY TEST BY THE MTT COLO RIMETRIC ASSAY FOR HUMAN SOLID TUMORS
- VernacularTitle:人实体瘤抗癌药物敏感试验MTT法的建立
- Author:
Weixue TANG
- Publication Type:Journal Article
- Keywords:
MTT colorimetr ic assay;
Tumor;
Anticancer drug sensitivity
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
We studied the methodology of 3-(4, 5-dimethyl-2-lhiazoly) )2, 5-diphenyl-2H-tetrazolium bromide(MTT) colorimetric assay for anticancer drug sensitivity test of human solid tumors. Results showed that the optimal cell concentration per well is 0.5-1 ? 10~5, the optimal incubation period for the action of anticancer drugs on tumor cells is three days. The concentration of anticancer drugs used should be varied with different drugs. We observed 167 samples of human solid tumors. The applicability of this test is concerned with the growth characteristics of tumor cells, histological types of malignancy, and the resected portion of the tumor specimen. The MTT colorimetric assay is a simple, rapid and reliable method for anticancer drug sensitivity test of human solid tumors
