PREPARATION AND APPLICATION OF A RAT ANTI-HRP MONOCLONAL ANTIBODY
- VernacularTitle:大鼠抗-HRP单克隆抗体的制备及应用的研究
- Author:
Sulian SUN
;
Lichun ZHAO
;
Xiaolan LIU
- Publication Type:Journal Article
- Keywords:
Rat hybridoma;
Rat anti-HRP McAb;
Rat McPAP;
ELISA;
Immunohistochemical assay
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The rat anti-HRP McAbs were prepared with rat-rat hybridoma and the rat McPAP complex was made by conjugating this McAb with HRP. The results of the present work indicated that the rat MePAP can be used for detection of antibodyantigen reaction by immunohistochemical techniques and ELISA. In both assay it gave strong staining reaction for positive antigen and with background staining appeared very pale. The sensitivity of rat McPAP and HRP labelling rabbit anti-rat IgG has been compared.
