IDENTIFICATION OF SEROTONIN-LIKE IMMUNOREACTIVE NEURONS IN NEURONAL CULTURES FROM E8 CHICK EMBRYO FOREBRAIN
- VernacularTitle:培养中E8鸡胚前脑5-羟色胺免疫反应神经细胞的鉴定
- Author:
Minghua ZHOU
- Publication Type:Journal Article
- Keywords:
Neuronal culture;
Neuron specific enolase;
Serotonin immunoreactivity;
Chick embryo;
Immunocytochemistry
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
The expression of serotonin-like immunoreactivity in day 4 neuronal cultures from E8 chick embryo forebrains was investigated by PAP immunocytochemical method. It was found that 98% of the dissociated forebrain cells cultured on laminin substratum were neuron specific enolase immunoreactive positive; and 10.4% of these neurons were mainly multipolar or bipolar and serotonin immunoreactive positive. In order to verify whether the remaining 2% NSE negative forebrain cells were neuroglial cells, we had used anti-glial fibrillary acid protein antibody to show that only 2% of the cultured forebrain cells were composed of GFAP immunoreactive positive cells, i. e. neuroglial cells.
