ULTRASTRUCTURE OF CARDIOMYOCYTES AND IMMUNOCYTOCHEMICAL LOCALIZATION OF ATRIAL NATRIURETIC PEPTIDE IN RAT ANTERIOR VENAE CAVAE
- VernacularTitle:大鼠前腔静脉心肌细胞的超微结构及其心房利钠多肽免疫细胞化学定位
- Author:
Chengying DING
- Publication Type:Journal Article
- Keywords:
Anterior venae cavae;
cardiomyocytes;
Specific granules;
Atrial natriuretic peptide;
Immunocytochemistry;
Rat
- From:
Acta Anatomica Sinica
1989;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
The wall of rat anterior venae cavae has cardiomyocytes running in different directions. These cardiomyocytes were connected by the intercalated discs. Some myocytes in the inner layer of the AVC near the atrium contained specific granules, which were very similar to those in the atrial myocytes in shape and distribution, but the number of the specific granules in the myocytes of venae was much less than that in the myocytes of atrium. Well-developed Golgi complexes, rough surfaced endoplasmic reticulum and multivesicular bodies were frequently found near the specific granules. Immuno-electron microscopy of the specific granules of the venae by using rabbit anti-synthetic rat ANP (1-28) serum, postembedding method and immunogold staining disclosed gold particles on the specific granules of myocytes in the venae were found in the atrial specific granules. It was also shown that the diameter of the labelled granules was somewhat related to the number of the labelling gold particles. No gold particles of the myocytes of the venae when the section was beforehand treated with the solution containing a combination of rat ANP and rabbit anti-ANP serum. The result indicated the presence of atrial natriuretic peptide (ANP) in the specific granules of myocytes of rat anterior venae cavae as well as in those of the atrium. The formation process of the specific granules and the possible way of the release of their contents were discussed.
