COMPONENT ANALYSIS OF ENZYMATICALLY DIGESTED HUMAN AMNIOTIC BASEMENT MEMBRANE WITH REFERENCE TO ITS ROLE IN SUPPORTING GROWTH OF CHICK EMBRYO FOREBRAIN NEURONS
- VernacularTitle:支持鸡胚前脑神经元生长的人羊膜基膜经酶消化后的成分分析
- Author:
Sijun LIN
;
Minghua ZHOU
- Publication Type:Journal Article
- Keywords:
SDS-polyacrylamide gel electrophoresis;
Human amniotic basement membrane;
Enzymatic digestion;
Forebrain neurons;
Laminin
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Cell culture and SDS-polyacrylamide gel electrophoresis (PAGE) methods were employed to analyse the role of human amniotic basement membrane (HABM) in supporting the growth of forebrain neurons and the components of HABM after enzymatic digestion. It was found that HABM treated with heparinase Ⅱ supported the growth of forebrain neurons 150% of that of the untreated HABM control. The results of SDS-PAGE analysis showed that HABM treated with heparinase Ⅱ had collagen-like polypeptides 2?_1 (260kD) and 2?_2 (225kD) reduced and lamininlike peptides (212kD and 200kD) increased; and that HABM treated with heparinase I or collagenase I had laminin reduced. This indicated that laminin is the major component in HABM to promote the growth of forebrain neurons.
