ISOLATION OF CELLS OF MLLERIAN DUCT AND ELECTRON IMMUNOCYTOCHEMICAL OBSERVATION ON THE RECEPTOR LOCALIZATION OF MLLERIAN INHIBITING SUBSTANCE
- VernacularTitle:苗勒管细胞的分离及苗勒管抑制素受体定位的免疫电镜观察
- Author:
Baikuan YE
- Publication Type:Journal Article
- Keywords:
Mllerian duct;
Mllerian inhibiting substance;
Cell culture;
Electron immunocytochemistry
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
An experimental study was carried out on the Mullerian duct cells, i.e. epithelial cells and stromal cells, treated with trypsin and isolated by Percoll gradient centrifugation. Then they were cultured separately. In order to observe the difference of cell death rate induced by Mullerian inhibiting substance (MIS) secreted from gonad, some cells were co-cultured with fetal testis or ovary in the same embryonic age. Other cells were used for immunocytochemistry at the electron microscopic level with anti-MIS serum as primary antibody and peroxidase labelled goat anti-rabbit IgG antiserum or streptavidin colloidal gold as secondary antibody to show the receptor localizations of MIS on epithelial cells and stromal cells. The results showed that the death rate of Mullerian duct epithelial cells induced by MIS secreted from testis was increased as compared with the control cells (P
