THE STUDY OF THE OPTIMUM CONDITION OF TWO FREEZING METHODS FOR PRESERVATION OF RABBIT EMBRYOS
- VernacularTitle:家兔胚胎两种冻存方法最佳条件的研究
- Author:
Yanrong WANG
;
Bin LIU
- Publication Type:Journal Article
- Keywords:
Rapid freezing;
Rabbit morula;
Cryoprotectant;
Embryo transfer;
Optimum condition
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
This paper reports the effects of the temperature control freezing and the liquid nitrogen vapour freezing on the viability of rabbit morulae. After rapid thawing, 70.27% embryos were able to develope well in vitro in the temperature control freezing group and in the liquid nitrogen vapour freezing group, the developmental rate was 80.56% when the freezing medium contained higher concentration of glycerol. After synchronous embryos were transplanted, the birth rate was 13.64% and 13.33%, respectively, in both freezing groups, and 11.11% in the control group. The results indicate that both freezing methods have almost the same good results, but the method of liquid nitrogen vapour freezing is more useful than the temperature control freezing method in some aspects.
