THE BIFURCATE PROJECTIONS OF MIDBRAIN PERIAQUEDUCTAL GRAY AND NUCLEUS RAPHE DORSALIS TO NUCLEUS RAPHE MAGNUS AND NUCLEUS ACCUMBENS IN THE RAT——A FLUORESCENCE DOUBLE-LABELED METHOD STUDY
- VernacularTitle:大鼠中脑中央灰质和中缝背核向伏核和中缝大核的分支投射——荧光素双标法研究
- Author:
Yunqing LI
;
Zhiren RAO
;
Jiwu SHI
- Publication Type:Journal Article
- Keywords:
Periaqueductal gray;
Nucleus raphe dorsalis;
Nucleus accumbens;
Nucleus raphe magnus;
Fluorescence double-labeled method;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the bifurcate projections of midbrain periaqueductal gray and nucleus raphe dorsalis to nucleus accumbens and nucleus raphe magnus, the fluorescence double-labeled method was used in the present study. Bisbenzimide (Bb) and propidium iodide (PI) were injected into nucleus raphe magnus and unilateral nucleus accumbens stereotaxically according to the time period necessary for their axonal transport. The percentages of double-labeled neurons were 21%; PI single labeled neurons were 32%; Bb single labeled neurons were 47%. Most of the labeled neurons were located in the middle and caudal parts of periaqueductal gray and the nucleus raphe dorsalis, and most were medium sized and fusiform and triangular in shape.