LIGHT AND ELECTRON MICROSCOPIC STUDIES ON HYBRIDS CROSSED BETWEEN RAT INTERMEDIATE OR LATE ERYTHROBLASTS AND MOUSE SP2/0 CELL LINE
- VernacularTitle:大鼠中幼、晚幼红细胞与小鼠浆细胞瘤SP 2/0细胞融合后的光镜和电镜观察
- Author:
Oingyi ZHANG
;
Yi TIAN
;
Shujie CUI
;
Shepu XUE
- Publication Type:Journal Article
- Keywords:
Cell fusion;
Intermediate or late erythroblast;
Mouse plasmocytoma SP2/0 cell;
Enucleation;
Denucleation factor;
Transmission electron microscopy;
Scanning electron microscopy
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The present study reported the observations with light and electronic micros copy on hybrid cells crossed between rat intermediate or late erythroblasts and mouse SP2/0 plasmocytoma cells. In a short period after fusion, the cell size and the ratio of nuclear heterochromatin in hybrid cells appeared to be increased, but the number of nucleoli, as well as the number of microvilli, finger-like processes, and nuclear cytoplasmic ratio were decreased. Swelling mitochondria, pycnotic nuclei and/or process of enucleation also could be seen in some hybrid cells. The subcul tured hybrid cells were characterized with less microvilli and cellular surface membrane processes, and showing marked changes in nuclear size, as well as the appearance of cytoplasmic vesicles and dense granules in some cells. The observations mentioned above provide morphological and ultrastructural evidences for the regulation of malignant phenotype of hybrid cell model we reported previously. The possible relationship between the deeancerization and the morphological changes of hybrid cell nucleus, cytoplasm and cell surface were briefly discussed.
